July 1, 2009
The Scientist , Jun 8 - You've unpacked your next-generation sequencing system and popped in some DNA or RNA. Five days later, you've sequenced 50 million tiny strings of nucleotides. Then what? Tolerating Mismatches USER: Nicholas Bergman, assistant professor of biology, Georgia Institute of Technology, Project: Mapping the transcriptome of anthrax-causing bacteria (Bacillus anthracis) and measuring gene expression levels Bergman's group uses Applied Biosystems' SOLiD gene sequencer, because it produces more data than do other new platforms... "It would take [an inaccuracy] and say this read is unmappable," Bergman says. The group needed a new algorithm that would tolerate these errors and move through vast amounts of data. (Full Story)
