The College of Sciences held its annual summer dinner on Sept. 18, hosted by Susan Lozier, the new dean and Betsy Middleton and John Clark Sutherland Chair of the College of Sciences. The gathering has become a tradition for welcoming new members; recognizing excellence in research, instruction, and service; and affirming the College’s special community of scholars.
Tim Cope, Christine Heitsch, and Marvin Whiteley received the 2019 Faculty Mentor Awards. Cope and Whiteley are professors in the School of Biological Sciences; Heitsch is a professor in the School of Mathematics.
Nominations for these awards come from early-career faculty. Nominators cite mentors’ willingness to make introductions, review proposals, and develop professional training programs as extremely helpful as they get familiar with and navigate their environment and roles.
"[Y]our recognition also shines a bright light on your school and the College of Sciences, for which we are grateful.”
Also celebrated at the 2019 Summer Dinner were recipients of distinguished faculty awards, funded through the generosity of alumni and friends.
Greg Blekherman, Martin Mourigal, and Ronghu Wu received the Cullen-Peck Fellowship Awards. These are made possible by a gift from alumni couple Frank Cullen and Libby Peck. The goal is to encourage the development of especially promising mid-career faculty. Bleckherman is an associate professor of mathematics, Mourigal is an assistant professor of physics, and Wu is an associate professor of chemistry and biochemistry.
Kim Cobb, professor of Earth and atmospheric sciences, received the 2019 Gretzinger Moving Forward Award. This is made possible by a gift from alumnus Ralph Gretzinger and his late wife, Jewel. The award recognizes leadership of a school chair or senior faculty member who has played a pivotal role in diversifying the composition of faculty, creating a family-friendly environment, and providing a supportive environment for early-career faculty.
Jennifer Glass, associate professor of Earth and atmospheric sciences, received the 2019 Eric R. Immel Memorial Award for Excellence in Teaching. This award is supported by an endowment fund given by alumnus Charles Crawford to recognize exemplary instruction of foundational courses.
“I am pleased that your distinction in research, teaching, and mentoring brings recognition your way,” Lozier said. “But your recognition also shines a bright light on your school and the College of Sciences, for which we are grateful.”
Lozier also welcomed faculty who joined in the 2019-20 academic year, herself included as professor of Earth and atmospheric sciences. Also present were Meghan Babcock and Keaton Fletcher, School of Psychology; Marcus Cicerone and Joshua Kretchmer, School of Chemistry and Biochemistry; and Glen Evenbly, School of Physics. Unable to attend were Alex Blumenthal, School of Mathematics, and Alonzo Whyte, School of Biological Sciences.
“As you set out on your academic journey, please know that we are here to support, mentor, and advise you along the way,” Lozier said. “Your good fortune will be ours as well.”
The Office of International Initiatives announces the launch of the Georgia Tech Guide for Responsible International Activities, a new online resource regarding guidelines, policies, and procedures around the Institute’s global activities and partnerships.
This summer, the Office of International Initiatives convened a working group of members of the Office of the Executive Vice President for Research and the Office of the Provost to develop a resource to guide educational and research activities that happen abroad. The major deliverables of the working group were designed to help Georgia Tech make decisions and ensure proper planning, compliance, and transparency around all international activities.
“Georgia Tech is proud to engage with researchers, scholars, and institutions all over the world as an expression of the Institute’s motto of Progress and Service,” said Chaouki T. Abdallah, Georgia Tech’s executive vice president for Research. “We remain wholeheartedly committed to those important global collaborations, but we must safeguard the Institute, and ensure all activities are fully transparent and in compliance with Georgia Tech policies, as well as applicable government laws and regulations.”
Site users can find direct links to Georgia Tech resources, policies, and relevant campus contacts for offices and units that manage a variety of issues, including export control; managing conflicts of interest; appointments at other institutions; intellectual property; materials, data, and confidential information; the Foreign Corrupt Practices Act (FCPA); international agreements; disclosing foreign relationships; lab tours; hosting foreign visitors; and international travel.
“Georgia Tech promotes a culture of global engagement and believes that our community is enriched through opportunities to study, work, serve, or do research abroad,” said Rafael L. Bras, provost and executive vice president for Academic Affairs. “Thanks to the working group, the guide now provides access to Tech’s standing policies and procedures governing international activities in one centralized location.”
The guide will be maintained by the Office of International Initiatives and will be available on faculty and staff resource pages at several touchpoints, including global.gatech.edu, research.gatech.edu, and provost.gatech.edu, among others.
The working group also refined Georgia Tech’s Guiding Principles for International Activities, a standard set of objective criteria used by the Office of International Initiatives for measuring each international activity’s impact on academic activities, value to the Institute, compliance with applicable policies, sustainability and viability, and risk assessment and mitigation concerns.
Georgia Tech is also in the process of creating an International Advisory Committee comprised of representatives of the administration, faculty, and staff. The committee will be chaired by Yves Berthelot, vice provost for International Initiatives, and will provide guidance and advice regarding how Georgia Tech engages internationally (e.g. research, MOUs, master research agreements, etc.).
“Our success in international activities must be assessed in full consideration of geopolitical factors, as well as current and potential state and federal regulations and legislation,” said Berthelot. “With those considerations in mind, the work of the committee will prove vital for Georgia Tech as we continue to grow our relationships across the world and explore new opportunities to engage globally.”
Nominations for the committee are currently being accepted through Oct. 7. Faculty and staff are encouraged to submit self-nominations or nominations for a colleague. Details on the final committee roster will be made available via the online tool, once finalized. To self-nominate or nominate a colleague for the committee, or for more information on the working group’s activities, contact Monique Tavares, director of Global Operations at mtavares@gatech.edu.
Editor's Note: This story by Nilde Maggie Dannreuther was originally published on Oct. 1, 2019, by the Gulf of Mexico Research Initiative. It is reposted here with permission.
Researchers at Florida State University and the Georgia Institute of Technology analyzed degradation processes of oil that was deposited along Gulf of Mexico beaches following Deepwater Horizon. They found that small millimeter-size oil particles and thin oil films that coated sand grains disappeared within a year, facilitated by the large surface-to-volume ratio of the small particles and films that allowed space for microbial colonization and biodegradation. In contrast, the degradation of golf-ball sized sediment-oil-agglomerates (SOAs or tarballs) with a smaller surface-to-volume ratio is a lengthier process.
Using a novel in-situ experimental setup, the researchers followed the degradation of buried SOAs for three years and, based on decay rates, estimated that the SOA decomposition would take about 30 years. The degradation of the same SOAs kept in a dark lab environment would take approximately 100 years, highlighting the key role of the beach environment and its microbial community in the oil degradation process.
The researchers published their findings in two studies, one in Marine Pollution Bulletin: Degradation of Deepwater Horizon oil buried in a Florida beach influenced by tidal pumping and one in Scientific Reports: Decomposition of sediment-oil-agglomerates in a Gulf of Mexico sandy beach.
Oil associated with Deepwater Horizon reached the Florida panhandle sandy beaches of the Florida panhandle on June 22, 2010. Waves generated by the distant passage of Hurricane Alex, deposited oil mousse high onto the beaches and strong winds blew an oily sea spray across the beach, coating the sands with oil.
Mixing of oil and sand in the swash zone produced large SOAs that were buried in the beach. The deposition of oil continued, and by the end of July, sands in the upper 70 cm of the beach were stained brown and veined by dark compacted layers of SOAs. Questions arose about the length of time that this oil would persist in Florida beaches.
To assess the degradation of the oil particles and oil films coating the sands, the teams of Markus Huettel and Joel Kostka quantified concentration changes of aliphatic and aromatic oil components; assessed microbial communities’ abundance, composition, and succession; and determined the transport of oxygen and carbon dioxide from June 2010-July 2011. To assess oil degradation in buried SOAs, the team conducted an in-situ experiment from October 2010-December 2013 using golf-ball size standardized SOAs that were embedded in the beach. They compared oil decomposition in buried SOAs to laboratory-incubated SOA material to determine the beach environment’s contribution to oil degradation.
Study author Markus Huettel explained the method for their in-situ experiment, “We combined and homogenized Deepwater Horizon SOAs that we collected at Pensacola Beach one week after the oil came to shore and filled the resulting SOA material in 100 golf-ball-size stainless steel teaballs. Five pairs of such standardized SOAs were attached to a vertical PVC pipe and buried in the beach, positioned at 10, 20, 30, 40 and 50 cm sediment depth, respectively. The ten arrays were removed from the beach one at a time over a period of 3 years. Using this method, we could follow the degradation of the SOAs at different sediment depths over time.”
Huettel emphasized the role of beaches as biocatalytical filters at the land-ocean interface, “Microbial degradation activities typically are most efficient when oxygen and warm temperatures are present, and this was supported by the tidal groundwater table oscillations in the beach. When the ebb tide sets in, the groundwater level in the beach drops, drawing air into the highly permeable beach sand. This ‘beach inhaling’ carries oxygen and heat into the sand, boosting the biodegradation activities within the beach. The rising groundwater table of the following flood acts like a piston pump, pushing air enriched in carbon dioxide out of the beach and moisture from deeper sands into the upper drier beach layers. This ‘beach exhaling’ is beneficial for the decomposition processes in the beach as gases resulting from the oil decomposition can reduce aerobic microbial degradation processes, and microbes need moisture to ‘drink.’ The beach, breathing in tidal rhythm, thus has similarities to an organism that aerobically ‘digests’ the buried oil, inhaling oxygen and exhaling carbon dioxide. After most oil had been decomposed, the microbial community of the beach reversed to a community typical to an unpolluted beach environment.”
Data for the study published in Marine Pollution Bulletin are archived at the National Center for Biotechnology Information (NCBI) under BioProject ID PRJNA294056 and publicly available through the Gulf of Mexico Research Initiative Information & Data Cooperative (GRIIDC) at DOI 10.7266/N7765CV9, DOI 10.7266/N7XW4HBZ, DOI 10.7266/N7BZ64J8, DOI 10.7266/N73J3BGD, DOI 10.7266/N7PZ56VV, DOI 10.7266/N7PG1Q83, DOI 10.7266/N7T72FZZ, DOI 10.7266/N78C9TSB, and DOI 10.7266/N7MG7N1S.
Data for the study published in Scientific Reports are publicly available through the Gulf of Mexico Research Initiative Information & Data Cooperative (GRIIDC) at DOI 10.7266/n7-wjj4-dq16, DOI 10.7266/n7-jjcn-y650, DOI 10.7266/n7-r0ca-f740, DOI 10.7266/n7-kzth-6056, DOI 10.7266/n7-jgbx-p395, and DOI 10.7266/n7-kavs-t279.
The Marine Pollution Bulletin study’s authors are Markus Huettel, Will A. Overholt, Joel E. Kostka, Christopher Hagan, John Kaba, Wm. Brian Wells, and Stacia Dudley.
The Scientific Reports study’s authors are Ioana Bociu, Boryoung Shin, Wm. Brian Wells, Joel E. Kostka, Konstantinos T. Konstantinidis, and Markus Huettel.
Editor's Note: This story by Nilde Maggie Dannreuther was originally published on Oct. 1, 2019, by the Gulf of Mexico Research Initiative. It is reposted here with permission.
Researchers at Florida State University and the Georgia Institute of Technology analyzed degradation processes of oil that was deposited along Gulf of Mexico beaches following Deepwater Horizon. They found that small millimeter-size oil particles and thin oil films that coated sand grains disappeared within a year, facilitated by the large surface-to-volume ratio of the small particles and films that allowed space for microbial colonization and biodegradation. In contrast, the degradation of golf-ball sized sediment-oil-agglomerates (SOAs or tarballs) with a smaller surface-to-volume ratio is a lengthier process.
Using a novel in-situ experimental setup, the researchers followed the degradation of buried SOAs for three years and, based on decay rates, estimated that the SOA decomposition would take about 30 years. The degradation of the same SOAs kept in a dark lab environment would take approximately 100 years, highlighting the key role of the beach environment and its microbial community in the oil degradation process.
The researchers published their findings in two studies, one in Marine Pollution Bulletin: Degradation of Deepwater Horizon oil buried in a Florida beach influenced by tidal pumping and one in Scientific Reports: Decomposition of sediment-oil-agglomerates in a Gulf of Mexico sandy beach.
Oil associated with Deepwater Horizon reached the Florida panhandle sandy beaches of the Florida panhandle on June 22, 2010. Waves generated by the distant passage of Hurricane Alex, deposited oil mousse high onto the beaches and strong winds blew an oily sea spray across the beach, coating the sands with oil.
Mixing of oil and sand in the swash zone produced large SOAs that were buried in the beach. The deposition of oil continued, and by the end of July, sands in the upper 70 cm of the beach were stained brown and veined by dark compacted layers of SOAs. Questions arose about the length of time that this oil would persist in Florida beaches.
To assess the degradation of the oil particles and oil films coating the sands, the teams of Markus Huettel and Joel Kostka quantified concentration changes of aliphatic and aromatic oil components; assessed microbial communities’ abundance, composition, and succession; and determined the transport of oxygen and carbon dioxide from June 2010-July 2011. To assess oil degradation in buried SOAs, the team conducted an in-situ experiment from October 2010-December 2013 using golf-ball size standardized SOAs that were embedded in the beach. They compared oil decomposition in buried SOAs to laboratory-incubated SOA material to determine the beach environment’s contribution to oil degradation.
Study author Markus Huettel explained the method for their in-situ experiment, “We combined and homogenized Deepwater Horizon SOAs that we collected at Pensacola Beach one week after the oil came to shore and filled the resulting SOA material in 100 golf-ball-size stainless steel teaballs. Five pairs of such standardized SOAs were attached to a vertical PVC pipe and buried in the beach, positioned at 10, 20, 30, 40 and 50 cm sediment depth, respectively. The ten arrays were removed from the beach one at a time over a period of 3 years. Using this method, we could follow the degradation of the SOAs at different sediment depths over time.”
Huettel emphasized the role of beaches as biocatalytical filters at the land-ocean interface, “Microbial degradation activities typically are most efficient when oxygen and warm temperatures are present, and this was supported by the tidal groundwater table oscillations in the beach. When the ebb tide sets in, the groundwater level in the beach drops, drawing air into the highly permeable beach sand. This ‘beach inhaling’ carries oxygen and heat into the sand, boosting the biodegradation activities within the beach. The rising groundwater table of the following flood acts like a piston pump, pushing air enriched in carbon dioxide out of the beach and moisture from deeper sands into the upper drier beach layers. This ‘beach exhaling’ is beneficial for the decomposition processes in the beach as gases resulting from the oil decomposition can reduce aerobic microbial degradation processes, and microbes need moisture to ‘drink.’ The beach, breathing in tidal rhythm, thus has similarities to an organism that aerobically ‘digests’ the buried oil, inhaling oxygen and exhaling carbon dioxide. After most oil had been decomposed, the microbial community of the beach reversed to a community typical to an unpolluted beach environment.”
Data for the study published in Marine Pollution Bulletin are archived at the National Center for Biotechnology Information (NCBI) under BioProject ID PRJNA294056 and publicly available through the Gulf of Mexico Research Initiative Information & Data Cooperative (GRIIDC) at DOI 10.7266/N7765CV9, DOI 10.7266/N7XW4HBZ, DOI 10.7266/N7BZ64J8, DOI 10.7266/N73J3BGD, DOI 10.7266/N7PZ56VV, DOI 10.7266/N7PG1Q83, DOI 10.7266/N7T72FZZ, DOI 10.7266/N78C9TSB, and DOI 10.7266/N7MG7N1S.
Data for the study published in Scientific Reports are publicly available through the Gulf of Mexico Research Initiative Information & Data Cooperative (GRIIDC) at DOI 10.7266/n7-wjj4-dq16, DOI 10.7266/n7-jjcn-y650, DOI 10.7266/n7-r0ca-f740, DOI 10.7266/n7-kzth-6056, DOI 10.7266/n7-jgbx-p395, and DOI 10.7266/n7-kavs-t279.
The Marine Pollution Bulletin study’s authors are Markus Huettel, Will A. Overholt, Joel E. Kostka, Christopher Hagan, John Kaba, Wm. Brian Wells, and Stacia Dudley.
The Scientific Reports study’s authors are Ioana Bociu, Boryoung Shin, Wm. Brian Wells, Joel E. Kostka, Konstantinos T. Konstantinidis, and Markus Huettel.
The Center for Microbial Dynamics and Infection will host a special recording of the podcast "This Week in Microbiology" (TWiM) on October 16, 2019. Sponsored by the American Society for Microbiology, TWiM is one of the most popular science podcasts in the country. The podcast will focus on one or two recent papers from CMDI members, who will join the hosts in discussing the work in front of an audience.
The podcast will focus on two recent papers by CMDI labs.
The first (Lewin et al. 2019, PNAS) presents the intriguing finding that an oral pathogen does better (higher fitness) in the wound environment when it infects alongside microbes from different (non-oral) environments, compared to microbes native to the oral environment. This effect appears to be related to an expanded metabolic repertoire provided by the non-natives, and is also tied to a core set of genes essential for success during co-infection.
The second (Beatty et al. 2019, Science Advances) focuses on a different system: the complex microbial community on corals. The study shows that the ability of coral water (sterilized washes of coral fragments) to suppress a known pathogen declines in corals on reefs subjected to overfishing compared to reefs in marine protected areas. This effect coincides with subtle differences in the coral microbiome.
Both studies highlight the importance of microbial interactions, a core theme of CMDI. Both are lead-authored by two young scientists: recently graduated CMDI graduate student Deanna Beatty and postdoctoral researcher Gina Lewin.
Event Details
"Implanted Neurotechnology to Understand and Restore Arm and Hand Function"
Jennifer Collinger, Ph.D.
Assistant Professor
Department of Physical Medicine & Rehabilitation
University of Pittsburgh
Watch via Livestream
ABSTRACT
After a cervical spinal cord injury, restoration of arm and hand function is a top rehabilitation priority. A brain-computer interface (BCI) can tap into sensorimotor information that remains intact in the brain and bypass the injured spinal cord to control an assistive device that may help the user regain function. Intracortical BCIs have allowed people to control reaching and grasping movements using their neural activity. However, BCIs users have primarily relied on visual feedback which may be insufficient for tasks involving object manipulation. Recently, we showed that intracortical microsimulation of somatosensory cortex can generate focal sensations that feel like they originate on the BCI users hand and that can be graded in intensity. Our current BCI study participant was able to use restored tactile sensations to improve performance on object transfer tasks. The time required to grasp the objects was reduced when ICMS feedback was provided as compared to visual feedback alone, likely due to increased certainty about the timing of object contact. Sensory feedback is particularly critical for dexterous and complex movements that are essential for many activities of daily living. We have also begun to investigate the role of motor cortex in modulating grasp force across a variety of task conditions. Across all experiments, we identified a large transient neural response during periods of active force application, as well as a tonic neural response during periods of constant force application. Interestingly, the relative strength of the transient and tonic neural components changes with task complexity suggesting a reallocation of cortical resources to accommodate the task requirements. This has implications for BCIs, which must account for contextual changes in order to maintain consistent performance.
BIO
Jennifer Collinger, PhD, is an Assistant Professor in the Department of Physical Medicine and Rehabilitation at the University of Pittsburgh and a Research Biomedical Engineer at the Pittsburgh VA R&D Center of Excellence. Dr. Collinger’s research interests are related to the use of neuroprosthetics to restore function for individuals with upper limb paralysis or loss. Specifically, she is developing intracortical brain-computer interface technology for individuals with tetraplegia. She also uses non-invasive imaging tools to study sensorimotor control and neuroplasticity after spinal cord injury or amputation.
The Bioengineering Seminar Series is co-hosted by the Parker H. Petit Institute for Bioengineering and Bioscience, and the Wallace H. Coulter Department of Biomedical Engineering at Georgia Tech and Emory University.
Event Details
Michael Borich, D.P.T., Ph.D.
Assistant Professor
Division of Rehabilitation Medicine
Emory University School of Medicine
“Perturbation-imaging Approaches to Study Functional Contributions of Cortical Activity to Human Movement”
The ability to learn and produce skilled movements is required for humans to successfully engage with each other and their environment. A principal role of the brain is to guide current, and plan future, movements based on past actions and potential rewards. In this talk, I will describe ongoing work in our lab employing multiple approaches to investigate the functional contributions of brain activity to normal and abnormal human movement. I will discuss how transcranial magnetic stimulation (TMS), a form of non-invasive brain stimulation, can be used both characterize and modulate cortical activity and connectivity during movement. I will also describe our recent findings showing abnormal TMS-evoked cortical reactivity post-stroke that is related to persistent paretic arm impairment. Lastly, I will discuss preliminary work applying alternative perturbation paradigms to study brain-behavior relationships in health and disease.
Event Details
NEW TWO-SPEAKER FORMAT for 2019-2020!
@ 8:30 A.M.
F. Levent Degertekin, Ph.D.
George W. Woodruff Chair in Mechanical Systems and Professor of Mechanical Engineering
Georgia Tech
"Microscale Systems and Sensors for Ultrasound and Magnetic Resonance Imaging"
ABSTRACT
Levent Degertekin's group performs modeling, design and implementation of microscale ultrasound devices and integrated systems for a broad range of medical applications spanning from catheter based intravascular and intracardiac ultrasound imaging systems, ultrasound imaging of the brain and skull, to acousto-optical sensors for safer magnetic resonance imaging (MRI).
RESEARCH
F. Levent Degertekin's research focuses on understanding of physical phenomena in acoustics and optics, and utilizing this knowledge creatively in the form of microfabricated devices. The research interests span several fields including atomic force microscopy (AFM), micromachined opto-acoustic devices, ultrasound imaging, bioanalytical instrumentation, and optical metrology. Degertekin's research group, in collaboration with an array of collaborators, has developed innovative devices for applications such as nanoscale material characterization and fast imaging, hearing aid microphones, intravascular imaging arrays for cardiology, bioanalytical mass spectrometry, and microscale parallel interferometers for metrology.
@ 9:00 A.M.
John Oshinski Ph.D.
Associate Professor
Wallace H. Coulter Department of Biomedical Engineering
Emory University and Georgia Tech
Associate Professor
Department of Radiology
Emory University School of Medicine
"Measuring Brain Tissue Motion and Cerebrovascular Blood Flow with MRI"
RESEARCH
John Oshinski's research is focused on developing cardiovascular imaging applications that directly impact patient care. His work includes development of cardiovascular imaging for understanding physiology, elucidating disease mechanisms, and evaluating treatment efficacy. Specific applications include development of cardiovascular magnetic resonance imaging (MRI) for assessing myocardial function and vascular hemodynamics.
Event Details
"Learning to Program Cellular Machines: Harnessing Cells to Treat Disease, Build Tissues, and Elucidate Design Principles"
Wendell Lim, Ph.D.
Professor of Cellular and Molecular Pharmacology
Biochemistry and Biophysics and Pharmaceutical Chemistry
Investigator, Howard Hughes Medical Institute
Byers Distinguished Professorship
University of California San Francisco
Abstract
The eras of molecular biology and genomics have given us an unprecedented picture of the molecular components underlying living systems. Nonetheless, our understanding of how these components are assembled to generate precision physiological systems remains far less developed. To approach this problem, we and others have been using synthetic biology methods to empirically explore how molecular components can be used to construct novel cellular functions. Using these approaches, we have shown that we can reprogram living cellular machines that carryout new novel precision functions, including therapeutic immune cells that precisely recognize and eliminate cancer cells, or cells that self-organize into tissues. By merging our understanding of cellular machines with our knowledge of our bodies and its diseases, we envision the development of a mature field of cell engineering with a toolbox of optimized molecular parts and circuit architectures, as well as disease and "GPS" sensors that allow the cell to target specific sites in the body or brain. Such a platform will allow us to rapidly and flexibly prototype and design cells that can identify sites of disease or degeneration and execute precisely targeted and controlled therapeutic actions.
Biography
Wendell Lim is the Byers Distinguished Professor and Chair of the Department of Cellular and Molecular Pharmacology at the University of California San Francisco, and an Investigator of the Howard Hughes Medical Institute. He received his A.B. in Chemistry, summa cum laude, from Harvard College, his Ph.D. in Biochemistry and Biophysics at the Massachusetts Institute of Technology and completed his postdoctoral training at Yale University. His research focuses on the design principles of molecular circuits that govern cell decision-making and responses. His lab has made contributions in understanding the molecular machinery of cell signaling and how molecular modules have been used in evolution to build novel new behaviors. Most recently he has been a pioneer in the emerging field of synthetic biology, exploring how these design principles can be harnessed to engineer cells with customized therapeutic response programs. He is an author of the textbook, Cell Signaling (Garland Science 2014) and was the founder of the cell therapy biotech startup, Cell Design Labs, which was acquired by Gilead Biosciences in 2017.
A community lunch will be served immediately following the presentation.
Event Details
“Specialized Properties of Adult Muscle Stem Cells”
Foteini Mourkioti, Ph.D.
Assistant Professor
Department of Orthopaedic Surgery
Perelman School of Medicine
University of Pennsylvania
Muscle stem cells play a major role in muscle regeneration. Following muscle injury, muscle stem cells become active, proliferate, and fuse with the surrounding tissue, allowing for the full regeneration within a few days. Pax7 is a marker of muscle stem cells that is conserved across many species, including humans. To look at muscle stem cells in vivo in a live animal, a Pax7EGFP mouse model was generated by inserting an enhanced green fluorescent protein (EGFP) cassette in the Pax7 gene (Tichy et. al., Skeletal Muscle, 2018). This allowed for the expression of EGFP driven by endogenous promoter and regulatory elements. Using 2-photon microscopy, we were able to visualize for the first time muscle stem cells in vivo and have identified unique and novel properties of these cells that have never been reported previously. Moreover, the study of muscle regeneration has primarily relied on retrospective analysis of static images from tissue section histology. Our system allows for longitudinal studies of muscle regeneration in a substantial reduced number of experimental animals and gives us the opportunity to test therapeutic interventions to reveal how early pathological symptoms correlate with later disease outcomes.
The Mourkioti lab has a long-term interest in understanding the fundamental aspects of skeletal muscle and cardiac function in normal or diseased conditions and in the practical aspects of manipulating these functions by using animal models and tissue engineering approaches for treatment intervention.
The Bioengineering Seminar Series is co-hosted by the Parker H. Petit Institute for Bioengineering and Bioscience, and the Wallace H. Coulter Department of Biomedical Engineering at Georgia Tech and Emory University.
